D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant W407F

Expression Data

Yield (mg/mL): 0.984

Kinetic Assay Data

**slope of W407F A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.16E-02	1.16E-02	1.16E-02
B	25.00	7.35E-03	7.68E-03	7.78E-03
C	8.33	2.40E-03	2.58E-03	2.75E-03
D	2.78	7.50E-04	7.50E-04	8.75E-04
E	0.93	4.50E-04	4.50E-04	4.75E-04
F	0.31	3.00E-04	2.50E-04	3.25E-04
G	0.10	3.25E-04	3.00E-04	2.50E-04
H	0.03		0.00E+00	0.00E+00

Yield (mg/mL): 0.984 Dilution Factor: 10×

Protein purified on 7/6/23 and assayed on 7/7/23.
Data uploaded by alexasvf and last updated on 2023-07-27 02:01:51.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	3.87E-01	3.82E-01	3.95E-01
B	25.00	3.27E-01	3.23E-01	3.24E-01
C	8.33	2.11E-01	2.00E-01	2.06E-01
D	2.78	9.90E-02	1.02E-01	1.18E-01
E	0.93	4.35E-02	4.32E-02	4.98E-02
F	0.31	1.35E-02	1.45E-02	1.64E-02
G	0.10	5.40E-03	4.95E-03	5.80E-03
H	0.03		0	0

Yield (mg/mL): 3.88 Dilution Factor: 100×

Protein purified on 7/6/23 and assayed on 7/7/23.
Data uploaded by alexasvf and last updated on 2023-07-27 01:11:37.

Design Data

Rosetta Score for W407F: 6.133

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.