D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant F253M

Expression Data

Yield (mg/mL): 3.346

Kinetic Assay Data

**slope of F253M A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	4.28E-01	4.26E-01	4.36E-01
B	25.00	3.25E-01	3.32E-01	3.43E-01
C	8.33	2.60E-01	2.64E-01	2.58E-01
D	2.78	1.52E-01	1.45E-01	1.51E-01
E	0.93	6.60E-02	6.27E-02	6.56E-02
F	0.31	2.30E-02	2.02E-02	2.34E-02
G	0.10	7.85E-03	6.75E-03	7.40E-03
H	0.03	0	0

Yield (mg/mL): 3.346 Dilution Factor: 100×

Protein purified on 7/20/23 and assayed on 7/21/23.
Data uploaded by alexasvf and last updated on 2023-07-27 02:40:20.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	5.45E-01	5.37E-01	5.34E-01
B	25.00	4.35E-01	4.03E-01	4.44E-01
C	8.33	3.13E-01	3.34E-01	3.24E-01
D	2.78	1.75E-01	1.82E-01	1.87E-01
E	0.93	8.68E-02	8.35E-02	9.28E-02
F	0.31	2.77E-02	2.77E-02	3.09E-02
G	0.10	9.20E-03	9.55E-03	9.55E-03
H	0.03	2.50E-04		3.00E-04

Yield (mg/mL): 5.176 Dilution Factor: 100×

Protein purified on 7/20/23 and assayed on 7/21/23.
Data uploaded by alexasvf and last updated on 2023-07-27 02:11:41.

Design Data

Rosetta Score for F253M: 6.856

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.