D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant E406Q

Expression Data

Yield (mg/mL): 4.458

Kinetic Assay Data

**slope of E406Q A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	5.17E-02	5.13E-02	5.19E-02
B	25.00	3.10E-02	3.21E-02	2.92E-02
C	8.33	1.52E-02	1.29E-02	1.32E-02
D	2.78	5.10E-03	6.30E-03	5.35E-03
E	0.93	2.05E-03	2.00E-03	2.40E-03
F	0.31	9.00E-04		1.05E-03
G	0.10	5.00E-04		5.50E-04
H	0.03	4.00E-04	4.00E-04

Yield (mg/mL): 4.458 Dilution Factor: 10×

Protein purified on 7/20/23 and assayed on 7/21/23.
Data uploaded by alexasvf and last updated on 2023-07-27 03:40:35.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	5.45E-01	5.37E-01	5.34E-01
B	25.00	4.35E-01	4.03E-01	4.44E-01
C	8.33	3.13E-01	3.34E-01	3.24E-01
D	2.78	1.75E-01	1.82E-01	1.87E-01
E	0.93	8.68E-02	8.35E-02	9.28E-02
F	0.31	2.77E-02	2.77E-02	3.09E-02
G	0.10	9.20E-03	9.55E-03	9.55E-03
H	0.03	2.50E-04		3.00E-04

Yield (mg/mL): 5.176 Dilution Factor: 100×

Protein purified on 7/20/23 and assayed on 7/21/23.
Data uploaded by alexasvf and last updated on 2023-07-27 02:11:41.

Design Data

Rosetta Score for E406Q: 4.305

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.