D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant T296Y

Expression Data

Yield (mg/mL): 4.352

Kinetic Assay Data

**slope of T296Y A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	6.20E-01	6.23E-01	6.35E-01
B	25.00	4.86E-01	4.72E-01	4.95E-01
C	8.33	3.53E-01	3.46E-01	3.58E-01
D	2.78	2.23E-01	2.26E-01	2.33E-01
E	0.93	1.08E-01	1.08E-01	1.16E-01
F	0.31	4.41E-02	4.33E-02	4.76E-02
G	0.10	1.61E-02	1.59E-02	1.71E-02
H	0.03		4.50E-04	4.00E-04

Yield (mg/mL): 4.352 Dilution Factor: 100×

Protein purified on 7/20/23 and assayed on 7/21/23.
Data uploaded by alexasvf and last updated on 2023-07-27 03:52:21.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	5.45E-01	5.37E-01	5.34E-01
B	25.00	4.35E-01	4.03E-01	4.44E-01
C	8.33	3.13E-01	3.34E-01	3.24E-01
D	2.78	1.75E-01	1.82E-01	1.87E-01
E	0.93	8.68E-02	8.35E-02	9.28E-02
F	0.31	2.77E-02	2.77E-02	3.09E-02
G	0.10	9.20E-03	9.55E-03	9.55E-03
H	0.03	2.50E-04		3.00E-04

Yield (mg/mL): 5.176 Dilution Factor: 100×

Protein purified on 7/20/23 and assayed on 7/21/23.
Data uploaded by alexasvf and last updated on 2023-07-27 02:11:41.

Design Data

Rosetta Score for T296Y: -1.076

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.