D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant C167D

Expression Data

Yield (mg/mL): 1.597

Kinetic Assay Data

**slope of C167D A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	2.25E-02	2.40E-02	2.51E-02
B	25.00	9.80E-03	1.12E-02	1.17E-02
C	8.33	6.45E-03	7.40E-03	7.20E-03
D	2.78	3.55E-03	2.95E-03	2.60E-03
E	0.93	1.50E-03	1.15E-03	1.40E-03
F	0.31	6.50E-04	8.00E-04	9.00E-04
G	0.10	4.50E-04	4.00E-04	4.50E-04
H	0.03	2.50E-04	3.00E-04	3.00E-04

Yield (mg/mL): 1.597 Dilution Factor: 100×

Protein purified on 7/27/23 and assayed on 7/28/23.
Data uploaded by tatumstevens and last updated on 2023-08-02 14:48:50.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	6.02E-01	6.71E-01	6.27E-01
B	25.00	4.86E-01	4.90E-01	4.43E-01
C	8.33	3.54E-01	3.52E-01	3.94E-01
D	2.78	2.37E-01	1.98E-01	2.38E-01
E	0.93		9.47E-02	1.07E-01
F	0.31	6.52E-02	3.56E-02	4.77E-02
G	0.10	2.43E-02	1.24E-02	1.82E-02
H	0.03	8.95E-03	4.75E-03	6.35E-03

Yield (mg/mL): 4.659 Dilution Factor: 100×

Protein purified on 7/27/23 and assayed on 7/28/23.
Data uploaded by tatumstevens and last updated on 2023-08-02 15:04:45.

Design Data

Rosetta Score for C167D: 7.612

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Justin B. Siegel: <jbsiegel@ucdavis.edu>.