D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant E177I

Expression Data

Yield (mg/mL): 2.604

Kinetic Assay Data

**slope of E177I A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.1763	0.1667
B	25.00	0.1537	0.1355
C	8.33	0.08175	0.04425	0.009651
D	2.78	0.0248	0.0225
E	0.93	0.0101	0.0085
F	0.31	0.00355	0.0034
G	0.10	0.0013	0.00125
H	0.03	0.00025	0.0003	0.00035

Yield (mg/mL): 2.604 Dilution Factor: 100×

Protein purified on 7/25/2023 and assayed on 7/26/2023.
Data uploaded by dripatel and last updated on 2023-08-03 14:27:43.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.398		0.4265
B	25.00	0.3119	0.2593	0.3378
C	8.33	0.0908	0.1275	0.1503
D	2.78	0.0183	0.06115	0.0851
E	0.93	0.0091	0.02505	0.03795
F	0.31	0.00455	0.0087	0.01345
G	0.10	0.0012	0.0032	0.005
H	0.03	0.0002	0.00035	0.0003

Yield (mg/mL): 4.192 Dilution Factor: 100×

Protein purified on 7/25/2023 and assayed on 7/26/2023.
Data uploaded by dripatel and last updated on 2023-08-03 15:15:08.

Design Data

Rosetta Score for E177I: 0.619

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.