Raw Data for BglB Variant A408S

Expression Data

SDS-PAGE
 0.232

Kinetic Assay Data

slope of A408S A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.0122 0.0099 0.0091
B 25.00 0.0067 0.0071 0.006
C 8.33 0.0058 0.0056 0.0063
D 2.78 0.0026 0.0024 0.0022
E 0.93 0.0012 0.0013
F 0.31 0.0007 0.0009 0.0008
G 0.10 0.0003 0.0003
H 0.03 0.00003 0
 0.232      100×

Protein purified on 10/11/2023 and assayed on 10/20/2023.
Data uploaded by SavannahBell and last updated on 2023-11-08 07:38:27.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.0506 0.0494 0.0471
B 25.00 0.0402 0.039 0.0375
C 8.33 0.0254 0.0266 0.0252
D 2.78 0.014 0.0148 0.0147
E 0.93 0.004 0.0051 0.0053
F 0.31 0.0022 0.0024 0.0026
G 0.10 0.0006 0.0005 0.0006
H 0.03 0 0 0
 0.6      100×

Protein purified on 10/11/2023 and assayed on 10/20/2023.
Data uploaded by hchayes0721 and last updated on 2023-11-08 07:44:52.


Thermostability Assay Data

slope of A408S A420 nm (min−1)
T (°C) 1 2 3
A 50.0 8.00E-05 1.00E-04 7.00E-05
B 48.3 1.00E-04 9.00E-05
C 45.7 2.00E-04 2.00E-04 2.00E-04
D 42.4 6.20E-03 8.20E-03 9.00E-03
E 37.7 3.30E-02 3.36E-02 3.65E-02
F 33.6 4.91E-02 4.43E-02 5.04E-02
G 31.3 5.59E-02 5.70E-02 5.75E-02
H 30.0 5.73E-02 5.70E-02 5.78E-02

Protein purified on 10/11/2023 and assayed on 10/20/2023.
Data uploaded by SavannahBell and last updated on 2023-11-08 07:55:30.


Reference Data for WT

slope of A408S A420 nm (min−1)
T (°C) 1 2 3
A 50.0 8.00E-05 1.00E-04 7.00E-05
B 48.3 1.00E-04 9.00E-05
C 45.7 2.00E-04 2.00E-04 2.00E-04
D 42.4 6.20E-03 8.20E-03 9.00E-03
E 37.7 3.30E-02 3.36E-02 3.65E-02
F 33.6 4.91E-02 4.43E-02 5.04E-02
G 31.3 5.59E-02 5.70E-02 5.75E-02
H 30.0 5.78E-02 5.70E-02 5.73E-02

Protein purified on 10/11/2023 and assayed on 10/20/2023.
Data uploaded by hchayes0721 and last updated on 2023-11-08 07:38:00.


Design Data

1.935

Citations

The unpublished data above were collected by students from Campbell University. For inquiries, please contact Evan Reynolds: <ereynolds@campbell.edu>.