D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant L434S

Expression Data

Yield (mg/mL): 2.493

Kinetic Assay Data

**slope of L434S A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	2.37E-01	0.2024	0.2078
B	25.00	1.67E-01	0.1821	0.1901
C	8.33	1.29E-01	0.1257	0.1461
D	2.78	1.14E-01	0.1283	0.1344
E	0.93	7.97E-02	0.09425	0.08895
F	0.31	9.57E-02	0.09105	8.04E-02
G	0.10	5.19E-02	0.05030	0.05520
H	0.03	6.50E-04	0.0009500	0.0004500

Yield (mg/mL): 2.493 Dilution Factor: 100×

Protein purified on 6/8/2023 and assayed on 6/12/2023.
Data uploaded by aidanhagerty and last updated on 2023-09-15 14:33:55.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.2486	0.2430	0.2310
B	25.00	0.2159	0.2037	0.1959
C	8.33	0.1619	0.1595	0.1499
D	2.78	0.1078	0.1266	0.1128
E	0.93	0.05745	0.05565	0.07065
F	0.31	0.03510	0.03080	0.04780
G	0.10	0.01460	0.01055	0.02280
H	0.03	6.00E-04	7.50E-04	8.00E-04

Yield (mg/mL): 2.893 Dilution Factor: 100×

Protein purified on 5/28/2023 and assayed on 6/1/2023.
Data uploaded by aidanhagerty and last updated on 2023-09-15 14:44:08.

Design Data

Rosetta Score for L434S: 1.207

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Justin B. Siegel: <jbsiegel@ucdavis.edu>.