D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant N157H

Expression Data

Yield (mg/mL): 2.059

Kinetic Assay Data

**slope of N157H A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.2239	0.2478	0.2284
B	25.00	0.1792	0.1950	0.1677
C	8.33	0.1272	0.1440	0.1561
D	2.78	0.1091	0.1086	0.1216
E	0.93	0.08070	0.07085	0.06360
F	0.31	0.06370	0.05520	0.04105
G	0.10	0.03300	0.03630
H	0.03	6.00E-04	6.00E-04

Yield (mg/mL): 2.059 Dilution Factor: 100×

Protein purified on 5/28/2023 and assayed on 6/1/2023.
Data uploaded by aidanhagerty and last updated on 2023-09-15 14:40:36.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.2486	0.2430	0.2310
B	25.00	0.2159	0.2037	0.1959
C	8.33	0.1619	0.1595	0.1499
D	2.78	0.1078	0.1266	0.1128
E	0.93	0.05745	0.05565	0.07065
F	0.31	0.03510	0.03080	0.04780
G	0.10	0.01460	0.01055	0.02280
H	0.03	6.00E-04	7.50E-04	8.00E-04

Yield (mg/mL): 2.893 Dilution Factor: 100×

Protein purified on 5/28/2023 and assayed on 6/1/2023.
Data uploaded by aidanhagerty and last updated on 2023-09-15 14:44:08.

Design Data

Rosetta Score for N157H: -0.398

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Justin B. Siegel: <jbsiegel@ucdavis.edu>.