Raw Data for BglB Variant E183D

Expression Data

SDS-PAGE
No expression

Kinetic Assay Data

slope of E183D A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 3.37E-01 3.83E-01 3.72E-01
B 25.00 3.28E-01 3.26E-01
C 8.33
D 2.78
E 0.93 1.68E-01 1.96E-01 1.71E-01
F 0.31 6.84E-02 6.47E-02 5.59E-02
G 0.10
H 0.03 3.31E-03 3.21E-03 3.93E-03
 2.66655E-5      100×

Protein purified on 2/28/24 and assayed on 3/14/24.
Data uploaded by zizi197 and last updated on 2024-04-18 06:56:30.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 2.76E-01 2.90E-01 2.76E-01
B 25.00 2.19E-01 2.25E-01 2.22E-01
C 8.33 2.03E-01 1.96E-01 1.93E-01
D 2.78 1.16E-01 1.18E-01 1.26E-01
E 0.93 5.84E-02 5.35E-02 5.89E-02
F 0.31 2.34E-02 2.51E-02 2.54E-02
G 0.10 1.10E-02 1.13E-02 1.15E-02
H 0.03 0 0 0
 1.0257      100×

Protein purified on 2/28/24 and assayed on 3/14/24.
Data uploaded by zizi197 and last updated on 2024-04-18 07:23:30.


Thermostability Assay Data

slope of E183D A420 nm (min−1)
T (°C) 1 2
A 30.0 1.26E-01 1.26E-01
B 30.5 1.59E-01 1.35E-01
C 31.7 1.63E-01 1.81E-01
D 33.2 0.19831 0.18259
E 35.4 0.17524 0.15952
F 38.3 0.12455 0.15021
G 41.7 0.031552 0.034034
H 44.5 0.0032069 0.0038276
I 46.8 0.0022759 0
J 48.3 0 0
K 49.5 0 0
L 50.0 0 0.0021724

Protein purified on 2/28/24 and assayed on 3/21/24.
Data uploaded by zizi197 and last updated on 2024-04-18 07:00:40.


Reference Data for WT

slope of E183D A420 nm (min−1)
T (°C) 1 2
A 30.0 2.68E-01 2.43E-01
B 30.5 2.24E-01 2.30E-01
C 31.7 2.60E-01 2.47E-01
D 33.2 0.22397 0.2219
E 35.4 0.20503 0.23079
F 38.3 0.24248 0.21724
G 41.7 0.087103 0.097345
H 44.5 0.0058966 0.0066207
I 46.8 0 0.0028966
J 48.3 0 0.0024828
K 49.5 0 0
L 50.0 0 0

Protein purified on 2/28/24 and assayed on 3/21/24.
Data uploaded by zizi197 and last updated on 2024-04-11 07:12:47.


Design Data

1.759

Citations

The unpublished data above were collected by students from Taylor University. For inquiries, please contact Daniel Kaluka: <daniel_kaluka@taylor.edu>.