D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant M149I

Expression Data

Yield (mg/mL): 3.148

Kinetic Assay Data

**slope of M149I A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.2987	0.2907	0.3022
B	25.00	0.2182	0.2313	0.2392
C	8.33	0.1159	0.1247	0.1353
D	2.78	0.06395	0.06065	0.0675
E	0.93	0.0227	0.02545	0.02795
F	0.31	0.0085	0.01	0.01035
G	0.10	0.00345	0.0037	0.00375
H	0.03	0.0014	0	0.00065

Yield (mg/mL): 3.148 Dilution Factor: 100×

Protein purified on 7/25/23 and assayed on 7/26/23.
Data uploaded by dripatel and last updated on 2023-09-30 15:22:30.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.398		0.4265
B	25.00	0.3119	0.2593	0.3378
C	8.33	0.0908	0.1275	0.1503
D	2.78	0.0183	0.06115	0.0851
E	0.93	0.0091	0.02505	0.03795
F	0.31	0.00455	0.0087	0.01345
G	0.10	0.0012	0.0032	0.005
H	0.03	0.0002	0.00035	0.0003

Yield (mg/mL): 4.192 Dilution Factor: 100×

Protein purified on 7/25/2023 and assayed on 7/26/2023.
Data uploaded by dripatel and last updated on 2023-08-03 15:15:08.

Design Data

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.