D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant S78A

Expression Data

Yield (mg/mL): 3.118

Kinetic Assay Data

**slope of S78A A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.40E-01	1.41E-01	1.38E-01
B	25.00	9.59E-02	8.37E-02	1.05E-01
C	8.33	5.37E-02	5.18E-02	5.24E-02
D	2.78	2.39E-02	2.28E-02	2.28E-02
E	0.93	9.05E-03	8.35E-03	7.65E-03
F	0.31	3.25E-03	3.05E-03	2.80E-03
G	0.10	1.65E-03	1.35E-03	1.05E-03
H	0.03		3.00E-04	3.00E-04

Yield (mg/mL): 3.118 Dilution Factor: 100×

Protein purified on 9/25/23 and assayed on 9/26/23.
Data uploaded by mvlouie and last updated on 2023-09-30 17:03:46.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	4.81E-01	4.59E-01	4.69E-01
B	25.00	3.94E-01	4.13E-01	4.17E-01
C	8.33	2.56E-01	2.76E-01	2.72E-01
D	2.78	1.46E-01	1.64E-01	1.53E-01
E	0.93	5.79E-02	7.33E-02	5.55E-02
F	0.31	2.03E-02		1.97E-02
G	0.10	6.65E-03		6.70E-03
H	0.03	3.50E-04	3.00E-04	3.50E-04

Yield (mg/mL): 4.546 Dilution Factor: 100×

Protein purified on 9/25/23 and assayed on 9/26/23.
Data uploaded by mvlouie and last updated on 2023-09-30 17:12:13.

Design Data

Rosetta Score for S78A: 1.921

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Michelle Louie: <mvlouie@ucdavis.edu>.