D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant W120M

Expression Data

Yield (mg/mL): 1.464

Kinetic Assay Data

**slope of W120M A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	2.56E-02	2.64E-02	2.62E-02
B	25.00	1.42E-02	1.36E-02	1.38E-02
C	8.33	6.45E-03	6.35E-03	6.30E-03
D	2.78	3.00E-03	3.05E-03	2.85E-03
E	0.93	1.20E-03	1.35E-03	1.20E-03
F	0.31	6.00E-04	6.50E-04	7.50E-04
G	0.10	2.50E-04	5.00E-04	4.00E-04
H	0.03	3.50E-04	3.50E-04

Yield (mg/mL): 1.464 Dilution Factor: 100×

Protein purified on 9/25/23 and assayed on 9/26/23.
Data uploaded by mvlouie and last updated on 2023-10-01 11:09:03.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	4.81E-01	4.59E-01	4.69E-01
B	25.00	3.94E-01	4.13E-01	4.17E-01
C	8.33	2.56E-01	2.76E-01	2.72E-01
D	2.78	1.46E-01	1.64E-01	1.53E-01
E	0.93	5.79E-02	7.33E-02	5.55E-02
F	0.31	2.03E-02		1.97E-02
G	0.10	6.65E-03		6.70E-03
H	0.03	3.50E-04	3.00E-04	3.50E-04

Yield (mg/mL): 4.546 Dilution Factor: 100×

Protein purified on 9/25/23 and assayed on 9/26/23.
Data uploaded by mvlouie and last updated on 2023-09-30 17:12:13.

Design Data

Rosetta Score for W120M: 5.232

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Michelle Louie: <mvlouie@ucdavis.edu>.