D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant N163G

Expression Data

Yield (mg/mL): 2.378

Kinetic Assay Data

**slope of N163G A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.43E-02	1.50E-02	1.56E-02
B	25.00	1.22E-02	1.21E-02	1.21E-02
C	8.33	7.55E-03	7.60E-03	7.95E-03
D	2.78	3.70E-03	3.90E-03	3.70E-03
E	0.93	1.55E-03	1.75E-03	1.60E-03
F	0.31	7.50E-04	7.50E-04	8.00E-04
G	0.10	3.50E-04	4.50E-04	5.50E-04
H	0.03	8.00E-04	0	2.50E-04

Yield (mg/mL): 2.378 Dilution Factor: 10×

Protein purified on 9/25/23 and assayed on 9/26/23.
Data uploaded by mvlouie and last updated on 2023-10-01 14:57:31.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	4.81E-01	4.59E-01	4.69E-01
B	25.00	3.94E-01	4.13E-01	4.17E-01
C	8.33	2.56E-01	2.76E-01	2.72E-01
D	2.78	1.46E-01	1.64E-01	1.53E-01
E	0.93	5.79E-02	7.33E-02	5.55E-02
F	0.31	2.03E-02		1.97E-02
G	0.10	6.65E-03		6.70E-03
H	0.03	3.50E-04	3.00E-04	3.50E-04

Yield (mg/mL): 4.546 Dilution Factor: 100×

Protein purified on 9/25/23 and assayed on 9/26/23.
Data uploaded by mvlouie and last updated on 2023-09-30 17:12:13.

Design Data

Rosetta Score for N163G: 5.965

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Michelle Louie: <mvlouie@ucdavis.edu>.