D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant T212E

Expression Data

Yield (mg/mL): 1.141

Kinetic Assay Data

**slope of T212E A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.145	0.1443	0.1365
B	25.00	0.1064	0.09965	0.0957
C	8.33	0.07755	0.07465	0.0885
D	2.78	0.0482	0.0496	0.06375
E	0.93	0.0275	0.03015	0.0361
F	0.31	0.0109	0.00945	0.01385
G	0.10	0.0049	0.00635	0.00705
H	0.03	0.00195	0.0021

Yield (mg/mL): 1.141 Dilution Factor: 100×

Protein purified on 8/29/23 and assayed on 8/30/23.
Data uploaded by enniferj and last updated on 2023-10-10 16:57:47.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.3006	0.2669	0.2839
B	25.00	0.239	0.2122	0.2238
C	8.33	0.1861	0.1664	0.1648
D	2.78	0.1088	0.1443	0.1156
E	0.93	0.06145		0.05445
F	0.31	0.0291		0.02455
G	0.10	0.0131		0.00975
H	0.03	0.00615		0.00355

Yield (mg/mL): 2.109 Dilution Factor: 100×

Protein purified on 8/29/23 and assayed on 8/30/23.
Data uploaded by enniferj and last updated on 2023-10-10 15:44:59.

Design Data

Rosetta Score for T212E: -0.21

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.