D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant N404Q

Expression Data

Yield (mg/mL): 0.569

Kinetic Assay Data

**slope of N404Q A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.00045	0.00045	0.00035
B	25.00	0.00035	0.0003	0.00035
C	8.33	0.00035	0.00025	0.0003
D	2.78	0.00025	0	0.0007001
E	0.93	0.0004	0.0002	0
F	0.31	0.00015	0.00015	0.00015
G	0.10
H	0.03

Yield (mg/mL): 0.569 Dilution Factor: 10×

Protein purified on 8/29/23 and assayed on 8/30/23.
Data uploaded by enniferj and last updated on 2023-10-10 17:14:33.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.3006	0.2669	0.2839
B	25.00	0.239	0.2122	0.2238
C	8.33	0.1861	0.1664	0.1648
D	2.78	0.1088	0.1443	0.1156
E	0.93	0.06145		0.05445
F	0.31	0.0291		0.02455
G	0.10	0.0131		0.00975
H	0.03	0.00615		0.00355

Yield (mg/mL): 2.109 Dilution Factor: 100×

Protein purified on 8/29/23 and assayed on 8/30/23.
Data uploaded by enniferj and last updated on 2023-10-10 15:44:59.

Design Data

Rosetta Score for N404Q: 0.603

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.