D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant E129Q

Expression Data

Yield (mg/mL): 0.899

Kinetic Assay Data

**slope of E129Q A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	9.13E-02	9.24E-02	9.14E-02
B	25.00	7.46E-02	7.06E-02	6.61E-02
C	8.33	5.50E-02	6.29E-02	5.61E-02
D	2.78	3.48E-02	3.60E-02	3.33E-02
E	0.93	1.43E-02	1.55E-02	1.32E-02
F	0.31	5.30E-03	5.95E-03	4.65E-03
G	0.10	1.80E-03	2.15E-03	1.75E-03
H	0.03	7.50E-04	1.00E-03	7.50E-04

Yield (mg/mL): 0.899 Dilution Factor: 100×

Protein purified on 10/19/23 and assayed on 10/23/23.
Data uploaded by mvlouie and last updated on 2023-10-26 15:07:14.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.59E-01	1.32E-01	1.27E-01
B	25.00	8.08E-02	9.99E-02	9.57E-02
C	8.33	7.24E-02	7.30E-02	7.36E-02
D	2.78	4.29E-02	4.40E-02	4.11E-02
E	0.93	1.86E-02	1.88E-02	1.73E-02
F	0.31	7.05E-03	7.10E-03	6.30E-03
G	0.10	2.45E-03	2.60E-03	2.40E-03
H	0.03	1.05E-03	1.05E-03	1.00E-03

Yield (mg/mL): 0.993 Dilution Factor: 100×

Protein purified on 10/19/23 and assayed on 10/23/23.
Data uploaded by mvlouie and last updated on 2023-10-26 13:56:26.

Design Data

Rosetta Score for E129Q: -1.11

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Michelle Louie: <mvlouie@ucdavis.edu>.