Raw Data for BglB Variant H315I

Expression Data

Kinetic Assay Data

Plate 20201217-4

slope of H315I A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	0.289221	0.283738	0.266736
	B	18.75	0.224991	0.221473	0.209299
	C	4.69	0.13194	0.11897	0.122565
	D	1.17	0.0526952	0.04422	0.0475703
	E	0.29	0.0161073	0.01302	0.0141667
	F	0.07	0.00469879	0.00430727	0.00418242
	G	0.02	0.00168242	0.00156121	0.0012297
	H	0.00	0.000993939	0.00187152	0.000561818

Yield / A_{280 nm}: 3.823     Dilution Factor:  100×

Download these data

Protein purified on 9/27/19 and assayed on 9/27/19.
Data uploaded by peishanh and last updated on 2022-06-27 07:04:03.

Reference Data for WT

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	8.86E-02	1.11E-01	8.31E-02
	B	18.75	7.30E-02	6.36E-02	5.65E-02
	C	4.69	5.06E-02	4.93E-02	4.65E-02
	D	1.17	2.51E-02	2.37E-02	2.22E-02
	E	0.29	9.62E-03	8.33E-03	8.61E-03
	F	0.07	2.82E-03	2.38E-03	2.40E-03
	G	0.02	7.90E-04	6.74E-04	7.26E-04
	H	0.00	1.88E-05	4.73E-05	0

Yield / A_{280 nm}: 1.03     Dilution Factor:  100×

Download these data

Protein purified on 9/21/19 and assayed on 9/21/19.
Data uploaded by peishanh and last updated on 2022-06-27 07:04:03.

Citations

The above data have been published in the following journal article(s):

• • Huang, P.; Chu, S.K.; Frizzo, H.N.; Connolly, M.P.; Caster, R.W.; & Siegel, J. B. (2020) “Evaluating Protein Engineering Thermostability Prediction Tools Using an Independently Generated Dataset..” ACS Omega 5(12), 6487–6493.