Raw Data for BglB Variant W325Y

Expression Data

SDS-PAGE
 3.02

Kinetic Assay Data

slope of W325Y A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 2.58E-02 2.91E-02 2.87E-02
B 25.00 2.72E-02 2.73E-02
C 8.33 2.43E-02 2.18E-02 2.24E-02
D 2.78 1.53E-02 1.61E-02
E 0.93 6.30E-03 7.95E-03
F 0.31 4.95E-03 4.20E-03 2.85E-03
G 0.10 1.20E-03 1.35E-03
H 0.03 4.50E-04 6.00E-04
 3.02      100×

Protein purified on 10/30/23 and assayed on 11/6/23.
Data uploaded by caromeilan24 and last updated on 2023-11-13 10:29:09.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 2.45E-01 2.40E-01 2.43E-01
B 25.00 2.05E-01 2.17E-01 2.04E-01
C 8.33 1.64E-01 1.62E-01 1.64E-01
D 2.78 1.47E-01 1.14E-01 1.16E-01
E 0.93 4.32E-02 4.10E-02 4.14E-02
F 0.31 1.65E-02 1.58E-02 1.55E-02
G 0.10 5.85E-03 5.85E-03 5.85E-03
H 0.03 6.00E-04 6.00E-04
 5.466      100×

Protein purified on 10/30/23 and assayed on 11/6/23.
Data uploaded by cgonz681 and last updated on 2023-11-13 10:25:48.


Thermostability Assay Data

slope of W325Y A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0 0.00E+00
B 48.3 1.05E-03 1.20E-03
C 45.7 4.50E-04 6.00E-04
D 42.4 1.95E-03 1.95E-03 1.95E-03
E 37.7 1.16E-02 1.14E-02 1.25E-02
F 33.6 1.20E-02 1.29E-02 1.35E-02
G 31.3 1.38E-02 1.37E-02 1.35E-02
H 30.0 1.38E-02 1.29E-02 1.29E-02

Protein purified on 10/30/23 and assayed on 11/6/23.
Data uploaded by caromeilan24 and last updated on 2023-11-13 10:25:48.


Reference Data for WT

slope of W325Y A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0 0 0
B 48.3 0 0
C 45.7 6.00E-04 4.50E-04
D 42.4 6.30E-03 5.85E-03 5.25E-03
E 37.7 1.06E-01 1.07E-01 1.04E-01
F 33.6 1.21E-01 1.19E-01 1.07E-01
G 31.3 1.19E-01 1.23E-01 1.22E-01
H 30.0 1.08E-01 1.12E-01 9.96E-02

Protein purified on 10/30/23 and assayed on 11/6/23.
Data uploaded by cgonz681 and last updated on 2023-11-13 10:31:34.


Design Data

5.139

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.