Raw Data for BglB Variant W325M

Expression Data

SDS-PAGE
 7.659

Kinetic Assay Data

slope of W325M A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 4.58E-02 0.04605 0.04531
B 25.00 0.042 0.0438 0.0402
C 8.33 2.61E-02 0.0318 0.03
D 2.78 0.02235 0.02145 0.01995
E 0.93 8.10E-03 6.60E-03 0.0069
F 0.31 1.95E-03 0.0027 0.00285
G 0.10 1.35E-03 0.0015 0.00135
H 0.03 0 0 0
 7.659      100×

Protein purified on 10/30/23 and assayed on 11/6/23.
Data uploaded by mateoomateoo and last updated on 2023-11-22 14:11:33.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 2.45E-01 2.40E-01 2.43E-01
B 25.00 2.05E-01 2.17E-01 2.04E-01
C 8.33 1.64E-01 1.62E-01 1.64E-01
D 2.78 1.47E-01 1.14E-01 1.16E-01
E 0.93 4.32E-02 4.10E-02 4.14E-02
F 0.31 1.65E-02 1.58E-02 1.55E-02
G 0.10 5.85E-03 5.85E-03 5.85E-03
H 0.03 6.00E-04 6.00E-04
 5.466      100×

Protein purified on 10/30/23 and assayed on 11/6/23.
Data uploaded by cgonz681 and last updated on 2023-11-13 10:25:48.


Thermostability Assay Data

slope of W325M A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0 0
B 48.3 0 0 0
C 45.7 0 0
D 42.4 0.00165 0.00165 0.00165
E 37.7 0.01695 0.01665 0.01725
F 33.6 0.02145 0.0225 0.0216
G 31.3 0.0249 0.024 0.0237
H 30.0 0.02445 0.0243 0.02475

Protein purified on 10/30/23 and assayed on 11/6/23.
Data uploaded by mateoomateoo and last updated on 2023-11-13 10:27:51.


Reference Data for WT

slope of W325M A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0 0 0
B 48.3 0 0
C 45.7 6.00E-04 4.50E-04
D 42.4 6.30E-03 5.85E-03 5.25E-03
E 37.7 1.06E-01 1.07E-01 1.04E-01
F 33.6 1.21E-01 1.19E-01 1.07E-01
G 31.3 1.19E-01 1.23E-01 1.22E-01
H 30.0 1.08E-01 1.12E-01 9.96E-02

Protein purified on 10/30/23 and assayed on 11/6/23.
Data uploaded by cgonz681 and last updated on 2023-11-13 10:31:34.


Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.