Raw Data for BglB Variant W325T

Expression Data

SDS-PAGE
 3.961

Kinetic Assay Data

slope of W325T A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 7.65E-03 7.35E-03 7.65E-03
B 25.00 8.70E-03 6.60E-03 8.40E-03
C 8.33 7.35E-03 7.05E-03
D 2.78 4.35E-03 4.65E-03 4.35E-03
E 0.93 2.10E-03 2.40E-03 2.55E-03
F 0.31 1.20E-03 1.65E-03 1.20E-03
G 0.10 9.00E-04 9.00E-04
H 0.03 0.00E+00 0.00E+00
 3.961      100×

Protein purified on 10/30/23 and assayed on 11/6/23.
Data uploaded by MariaPuello and last updated on 2023-11-13 10:28:32.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 2.45E-01 2.40E-01 2.43E-01
B 25.00 2.05E-01 2.17E-01 2.04E-01
C 8.33 1.64E-01 1.62E-01 1.64E-01
D 2.78 1.47E-01 1.14E-01 1.16E-01
E 0.93 4.32E-02 4.10E-02 4.14E-02
F 0.31 1.65E-02 1.58E-02 1.55E-02
G 0.10 5.85E-03 5.85E-03 5.85E-03
H 0.03 6.00E-04 6.00E-04
 5.466      100×

Protein purified on 10/30/23 and assayed on 11/6/23.
Data uploaded by cgonz681 and last updated on 2023-11-13 10:25:48.


Thermostability Assay Data

slope of W325T A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0 0
B 48.3 0 0 0
C 45.7 0 0
D 42.4 9.00E-04 9.00E-04 7.50E-04
E 37.7 3.90E-03 4.50E-03 3.60E-03
F 33.6 4.05E-03 4.20E-03 4.20E-03
G 31.3 4.65E-03 4.80E-03 4.80E-03
H 30.0 5.70E-03 5.70E-03 6.15E-03

Protein purified on 10/30/23 and assayed on 11/6/23.
Data uploaded by MariaPuello and last updated on 2023-11-13 10:29:24.


Reference Data for WT

slope of W325T A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0 0 0
B 48.3 0 0
C 45.7 6.00E-04 4.50E-04
D 42.4 6.30E-03 5.85E-03 5.25E-03
E 37.7 1.06E-01 1.07E-01 1.04E-01
F 33.6 1.21E-01 1.19E-01 1.07E-01
G 31.3 1.19E-01 1.23E-01 1.22E-01
H 30.0 1.08E-01 1.12E-01 9.96E-02

Protein purified on 10/30/23 and assayed on 11/6/23.
Data uploaded by cgonz681 and last updated on 2023-11-13 10:31:34.


Design Data

8.95

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.