D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant W325I

Expression Data

Yield (mg/mL): 3.45

Kinetic Assay Data

**slope of W325I A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	6.30E-03	6.15E-03	6.00E-03
B	25.00	6.00E-03	6.60E-03
C	8.33	5.70E-03	5.55E-03	5.85E-03
D	2.78	3.45E-03	4.50E-03	4.20E-03
E	0.93	2.40E-03	2.55E-03	2.70E-03
F	0.31	1.50E-03	1.35E-03	1.65E-03
G	0.10	9.00E-04	7.50E-04	1.05E-03
H	0.03		7.50E-04	6.00E-04

Yield (mg/mL): 3.45 Dilution Factor: 100×

Protein purified on 10/30/23 and assayed on 11/6/23.
Data uploaded by ttruong and last updated on 2023-11-13 09:47:11.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	5.90E-02	5.49E-02	5.25E-02
B	25.00	5.16E-02	4.65E-02	4.53E-02
C	8.33	3.47E-02	3.36E-02	3.32E-02
D	2.78	2.21E-02	2.03E-02	2.04E-02
E	0.93	1.02E-02	9.90E-03	9.00E-03
F	0.31	4.05E-03	3.60E-03	3.90E-03
G	0.10	1.80E-03	1.35E-03	1.95E-03
H	0.03	0.00E+00	6.00E-04	9.00E-04

Yield (mg/mL): 1.017 Dilution Factor: 100×

Protein purified on 10/30/23 and assayed on 11/6/23.
Data uploaded by PamelaSilveyra and last updated on 2023-11-13 09:49:40.

Thermostability Assay Data

**slope of W325I A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	7.50E-04	6.00E-04	9.00E-04
B	48.3		0	6.00E-04
C	45.7	6.00E-04		4.50E-04
D	42.4	7.50E-04	6.00E-04	6.00E-04
E	37.7	3.15E-03	3.15E-03	3.00E-03
F	33.6	3.60E-03	3.75E-03	3.45E-03
G	31.3	4.35E-03	4.05E-03	3.60E-03
H	30.0	4.05E-03	3.90E-03	3.45E-03

Protein purified on 10/30/23 and assayed on 11/6/23.
Data uploaded by ttruong and last updated on 2023-11-13 10:09:11.

Reference Data for WT

**slope of W325I A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0		0	0
B	48.3		6.00E-04	4.50E-04
C	45.7		0	0
D	42.4	3.15E-03	3.15E-03	3.15E-03
E	37.7	1.25E-02	1.37E-02	1.20E-02
F	33.6	1.64E-02	1.64E-02	1.62E-02
G	31.3	1.82E-02	1.65E-02	1.59E-02
H	30.0	1.86E-02	1.74E-02	1.61E-02

Protein purified on 10/30/23 and assayed on 11/6/23.
Data uploaded by PamelaSilveyra and last updated on 2023-11-13 10:09:32.

Design Data

Rosetta Score for W325I: 29.698

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.