D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant W325S

Expression Data

Yield (mg/mL): 1.893

Kinetic Assay Data

**slope of W325S A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	3.15E-03	3.15E-03	3.60E-03
B	25.00	3.45E-03	3.45E-03	3.30E-03
C	8.33	2.85E-03	3.00E-03	2.85E-03
D	2.78	3.45E-03	2.55E-03	2.25E-03
E	0.93	1.20E-03	1.20E-03	1.35E-03
F	0.31	9.00E-04	9.00E-04	9.00E-04
G	0.10	4.50E-04	7.50E-04	6.00E-04
H	0.03	7.50E-04	0.00E+00	4.50E-04

Yield (mg/mL): 1.893 Dilution Factor: 100×

Protein purified on 10/30/23 and assayed on 11/6/23.
Data uploaded by angelinarivas and last updated on 2023-11-13 10:17:31.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.07410	0.07965	0.07845
B	25.00	0.07020	0.06135	0.06210
C	8.33	0.04620	0.04380	0.04830
D	2.78	0.02715	0.02970	0.02715
E	0.93	0.01620	0.01425	0.01245
F	0.31	0.006000	0.004950	0.005250
G	0.10	0.002850	0.001800	0.002250
H	0.03	0.0004500	0.001650	0.0000000

Yield (mg/mL): 1.677 Dilution Factor: 100×

Protein purified on 10/30/2023 and assayed on 11/6/2023.
Data uploaded by kli023 and last updated on 2023-11-13 10:13:09.

Thermostability Assay Data

**slope of W325S A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0		0	0
B	48.3	1.05E-03	1.05E-03
C	45.7	7.50E-04	6.00E-04	1.05E-03
D	42.4	9.00E-04	7.50E-04	7.50E-04
E	37.7	1.05E-03	1.20E-03	7.50E-04
F	33.6	1.35E-03	1.50E-03	1.20E-03
G	31.3	1.50E-03	1.35E-03	1.20E-03
H	30.0	1.50E-03	2.40E-03	1.20E-03

Protein purified on 10/30/23 and assayed on 11/6/23.
Data uploaded by angelinarivas and last updated on 2023-11-13 10:34:12.

Reference Data for WT

**slope of W325S A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	0.0000000		0.0000000
B	48.3		0.0004500	0.0006000
C	45.7	0.0007500	0.0006000	0.0006000
D	42.4	0.002250	0.002250	0.001800
E	37.7	0.01710	0.01695	0.01605
F	33.6	0.02730	0.02805	0.02475
G	31.3	0.02865	0.02475	0.02535
H	30.0	0.02580	0.03060	0.02835

Protein purified on 10/30/2023 and assayed on 11/6/2023.
Data uploaded by kli023 and last updated on 2023-11-13 10:13:19.

Design Data

Rosetta Score for W325S: 7.181

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.