Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 1.017

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	5.90E-02	5.49E-02	5.25E-02
	B	25.00	5.16E-02	4.65E-02	4.53E-02
	C	8.33	3.47E-02	3.36E-02	3.32E-02
	D	2.78	2.21E-02	2.03E-02	2.04E-02
	E	0.93	1.02E-02	9.90E-03	9.00E-03
	F	0.31	4.05E-03	3.60E-03	3.90E-03
	G	0.10	1.80E-03	1.35E-03	1.95E-03
	H	0.03	0.00E+00	6.00E-04	9.00E-04

Yield (mg/mL): 1.017     Dilution Factor:  100×

Download these data

Protein purified on 10/30/23 and assayed on 11/6/23.
Data uploaded by PamelaSilveyra and last updated on 2023-11-13 09:49:40.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0		0	0
	B	48.3		6.00E-04	4.50E-04
	C	45.7		0	0
	D	42.4	3.15E-03	3.15E-03	3.15E-03
	E	37.7	1.25E-02	1.37E-02	1.20E-02
	F	33.6	1.64E-02	1.64E-02	1.62E-02
	G	31.3	1.82E-02	1.65E-02	1.59E-02
	H	30.0	1.86E-02	1.74E-02	1.61E-02

Download these data

Protein purified on 10/30/23 and assayed on 11/6/23.
Data uploaded by PamelaSilveyra and last updated on 2023-11-13 10:09:32.

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.