Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 1.677

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	0.07410	0.07965	0.07845
	B	25.00	0.07020	0.06135	0.06210
	C	8.33	0.04620	0.04380	0.04830
	D	2.78	0.02715	0.02970	0.02715
	E	0.93	0.01620	0.01425	0.01245
	F	0.31	0.006000	0.004950	0.005250
	G	0.10	0.002850	0.001800	0.002250
	H	0.03	0.0004500	0.001650	0.0000000

Yield (mg/mL): 1.677     Dilution Factor:  100×

Download these data

Protein purified on 10/30/2023 and assayed on 11/6/2023.
Data uploaded by kli023 and last updated on 2023-11-13 10:13:09.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	0.0000000		0.0000000
	B	48.3		0.0004500	0.0006000
	C	45.7	0.0007500	0.0006000	0.0006000
	D	42.4	0.002250	0.002250	0.001800
	E	37.7	0.01710	0.01695	0.01605
	F	33.6	0.02730	0.02805	0.02475
	G	31.3	0.02865	0.02475	0.02535
	H	30.0	0.02580	0.03060	0.02835

Download these data

Protein purified on 10/30/2023 and assayed on 11/6/2023.
Data uploaded by kli023 and last updated on 2023-11-13 10:13:19.

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.