Raw Data for BglB Variant W325E

Expression Data

Yield (mg/mL): 3.581

Kinetic Assay Data

slope of W325E A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	1.50E-02		1.56E-02
	B	25.00
	C	8.33	1.56E-02	1.46E-02	1.46E-02
	D	2.78	9.15E-03	8.55E-03	8.85E-03
	E	0.93	4.35E-03	4.05E-03	4.50E-03
	F	0.31	1.95E-03	2.10E-03	2.10E-03
	G	0.10	1.20E-03	1.05E-03	1.35E-03
	H	0.03	7.50E-04		9.00E-04

Yield (mg/mL): 3.581     Dilution Factor:  10×

Download these data

Protein purified on 10/30/23 and assayed on 11/6/23.
Data uploaded by xaviercalvo and last updated on 2023-11-13 10:21:22.

Reference Data for WT

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	0.2694	0.273	0.2429
	B	25.00	0.2583	0.2281	0.2228
	C	8.33	0.156	0.1848	0.1539
	D	2.78	0.1095	0.1068	0.1049
	E	0.93	0.04156	0.04575	0.04365
	F	0.31	0.0168	0.0156	0.0183
	G	0.10	0.0066	0.00615	0.00615
	H	0.03	0.0006		0.0006

Yield (mg/mL): 4.542     Dilution Factor:  100×

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Protein purified on 10/30/23 and assayed on 11/6/23.
Data uploaded by circez and last updated on 2023-11-13 09:48:50.

Thermostability Assay Data

slope of W325E A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	0		0
	B	48.3	0	0
	C	45.7		6.00E-04	6.00E-04
	D	42.4	1.05E-03	7.50E-04	0
	E	37.7	9.45E-03	8.40E-03	7.50E-03
	F	33.6	1.35E-02	1.31E-02	1.31E-02
	G	31.3	1.65E-02	1.38E-02	1.31E-02
	H	30.0	1.44E-02	1.41E-02	1.26E-02

Download these data

Protein purified on 10/30/23 and assayed on 11/6/23.
Data uploaded by xaviercalvo and last updated on 2023-11-13 10:15:26.

Reference Data for WT

Wild-type data not found. It may have been rejected in the curation process and deleted from the database.

Design Data

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.