Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 5.466

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	2.45E-01	2.40E-01	2.43E-01
	B	25.00	2.05E-01	2.17E-01	2.04E-01
	C	8.33	1.64E-01	1.62E-01	1.64E-01
	D	2.78	1.47E-01	1.14E-01	1.16E-01
	E	0.93	4.32E-02	4.10E-02	4.14E-02
	F	0.31	1.65E-02	1.58E-02	1.55E-02
	G	0.10	5.85E-03	5.85E-03	5.85E-03
	H	0.03		6.00E-04	6.00E-04

Yield (mg/mL): 5.466     Dilution Factor:  100×

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Protein purified on 10/30/23 and assayed on 11/6/23.
Data uploaded by cgonz681 and last updated on 2023-11-13 10:25:48.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	0	0	0
	B	48.3	0	0
	C	45.7	6.00E-04	4.50E-04
	D	42.4	6.30E-03	5.85E-03	5.25E-03
	E	37.7	1.06E-01	1.07E-01	1.04E-01
	F	33.6	1.21E-01	1.19E-01	1.07E-01
	G	31.3	1.19E-01	1.23E-01	1.22E-01
	H	30.0	1.08E-01	1.12E-01	9.96E-02

Download these data

Protein purified on 10/30/23 and assayed on 11/6/23.
Data uploaded by cgonz681 and last updated on 2023-11-13 10:31:34.

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.