Raw Data for BglB Variant N293T

Expression Data

SDS-PAGE
No expression

Kinetic Assay Data

slope of N293T A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.0153 0.0165 0.01635
B 25.00 0.01005 0.0108 0.01155
C 8.33 0.0069 0.0069 0.0075
D 2.78 0.00375 0.0033 0.0036
E 0.93 0.0021 0.00195 0.00165
F 0.31 0.00135 0.00105 0.0012
G 0.10 0.0009 0.0009 0.00105
H 0.03 0.00045 0.00045
 2.111      100×

Protein purified on 10/30/23 and assayed on 11/6/23.
Data uploaded by MaiaHadeed and last updated on 2023-11-13 13:56:52.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 2.18E-01 2.41E-01 2.30E-01
B 25.00 1.72E-01 1.83E-01 1.84E-01
C 8.33 1.52E-01 1.59E-01 1.61E-01
D 2.78 9.78E-02 1.13E-01 9.99E-02
E 0.93 4.92E-02 5.99E-02 5.15E-02
F 0.31 2.21E-02 2.72E-02 2.54E-02
G 0.10 8.70E-03 1.26E-02 1.07E-02
H 0.03 1.65E-03 1.65E-03 1.65E-03
 5.041      100×

Protein purified on 10/30/23 and assayed on 11/6/23.
Data uploaded by NicolasFuentes and last updated on 2023-11-13 14:06:10.


Thermostability Assay Data

slope of N293T A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0 0
B 48.3 0.0009 0.0007502
C 45.7 0.0006 0.0006
D 42.4 0.0006 0.00105 0.0009
E 37.7 0.00285 0.003 0.00255
F 33.6 0.00375 0.00375
G 31.3 0.0039 0.00405 0.0036
H 30.0 0.005099 0.00495 0.00435

Protein purified on 10/30/23 and assayed on 11/6/23.
Data uploaded by MaiaHadeed and last updated on 2023-11-13 14:17:59.


Reference Data for WT

slope of N293T A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0 0 0
B 48.3 1.05E-03 6.00E-04
C 45.7 1.05E-03 6.00E-04 7.50E-04
D 42.4 8.55E-03 8.55E-03
E 37.7 9.38E-02 1.03E-01 7.55E-02
F 33.6 1.12E-01 1.12E-01 8.67E-02
G 31.3 1.11E-01 1.11E-01 8.85E-02
H 30.0 1.13E-01 1.02E-01

Protein purified on 10/30/23 and assayed on 11/6/23.
Data uploaded by NicolasFuentes and last updated on 2024-08-08 18:51:22.


Design Data

11.782

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.