D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant H178C

Expression Data

Yield (mg/mL): 0.16

Kinetic Assay Data

**slope of H178C A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	4.60E-03	5.10E-03	5.10E-03
B	25.00	4.20E-03	4.20E-03	4.20E-03
C	8.33	2.90E-03	3.00E-03	3.00E-03
D	2.78	1.50E-03	1.60E-03	1.60E-03
E	0.93	6.00E-04	7.00E-04	6.00E-04
F	0.31	2.00E-04	2.00E-04	2.00E-04
G	0.10	9.00E-05	9.00E-05	9.00E-05
H	0.03	9.00E-05		8.00E-05

Yield (mg/mL): 0.16 Dilution Factor: 100×

Protein purified on 10/19/23 and assayed on 11/8/23.
Data uploaded by khudick and last updated on 2025-02-11 14:26:42.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	6.10E-02	6.06E-02	6.36E-02
B	25.00	5.18E-02	5.10E-02	4.70E-02
C	8.33	3.76E-02	3.77E-02	3.63E-02
D	2.78	2.18E-02	2.14E-02
E	0.93	6.10E-03	6.80E-03	6.90E-03
F	0.31	2.40E-03	2.60E-03	2.50E-03
G	0.10	9.00E-04	1.00E-03	9.00E-04
H	0.03		0.00E+00	0.00E+00

Yield (mg/mL): 0.307 Dilution Factor: 100×

Protein purified on 10/19/23 and assayed on 11/8/23.
Data uploaded by khudick and last updated on 2025-02-11 14:20:12.

Design Data

Rosetta Score for H178C: 3.432

Citations

The unpublished data above were collected by students from Colorado State University. For inquiries, please contact Carolina Mehaffy: <carolina.mehaffy@colostate.edu>.