Raw Data for BglB Variant C198A

Expression Data

SDS-PAGE
 3.026

Kinetic Assay Data

slope of C198A A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 7.29E-02 7.02E-02 7.55E-02
B 25.00 6.27E-02 5.94E-02 5.78E-02
C 8.33 4.43E-02 4.88E-02 4.40E-02
D 2.78 2.87E-02 2.39E-02 2.55E-02
E 0.93 1.94E-02 1.70E-02 1.56E-02
F 0.31 7.95E-03 8.55E-03 7.20E-03
G 0.10 3.30E-03 2.85E-03 3.00E-03
H 0.03 0 0 0
 3.026      100×

Protein purified on 10/27/23 and assayed on 11/3/23.
Data uploaded by Castilla34 and last updated on 2024-05-09 11:33:01.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 7.07E-02 7.20E-02 6.98E-02
B 25.00 5.54E-02 5.30E-02 5.42E-02
C 8.33 4.85E-02 4.97E-02 4.94E-02
D 2.78 3.42E-02 3.24E-02 2.94E-02
E 0.93 2.04E-02 2.09E-02 1.64E-02
F 0.31 8.85E-03 8.55E-03 7.20E-03
G 0.10 4.80E-03 4.20E-03 3.30E-03
H 0.03 9.00E-04 7.50E-04
 2.03      100×

Protein purified on 10/27/23 and assayed on 11/3/23.
Data uploaded by CaitlynR and last updated on 2023-11-17 10:17:43.


Thermostability Assay Data

slope of C198A A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0 0
B 48.3 0 0
C 45.7 9.00E-04 7.50E-04
D 42.4 8.25E-03 8.10E-03 7.95E-03
E 37.7 2.97E-02 2.48E-02 2.48E-02
F 33.6 3.75E-02 3.50E-02
G 31.3 3.95E-02 3.71E-02 3.51E-02
H 30.0 4.02E-02 3.81E-02 3.42E-02

Protein purified on 10/27/23 and assayed on 11/3/23.
Data uploaded by Castilla34 and last updated on 2024-05-09 11:33:58.


Reference Data for WT

slope of C198A A420 nm (min−1)
T (°C) 1 2 3
A 50.0 7.50E-04 6.00E-04 6.00E-04
B 48.3 7.50E-04 7.50E-04
C 45.7 1.05E-03 7.50E-04 6.00E-04
D 42.4 6.45E-03 6.15E-03 6.30E-03
E 37.7 3.36E-02 3.24E-02 2.55E-02
F 33.6 3.36E-02 3.03E-02 3.00E-02
G 31.3 5.16E-02 4.04E-02 4.07E-02
H 30.0 3.77E-02 4.31E-02 3.75E-02

Protein purified on 10/27/23 and assayed on 11/3/23.
Data uploaded by CaitlynR and last updated on 2023-11-17 10:48:03.


Design Data

-0.89

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.