Raw Data for BglB Variant C198M

Expression Data

SDS-PAGE
 1.244

Kinetic Assay Data

slope of C198M A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.02835 0.02925 0.03270
B 25.00 0.02281 0.02025 0.02445
C 8.33 0.01815 0.01635 0.01950
D 2.78 0.01215 0.01200 0.01170
E 0.93 0.005400 0.005400
F 0.31 0.003000 0.002850 0.002100
G 0.10 0.001050 0.001200 0.001050
H 0.03 0.00E+00 0.00E+00 0.00E+00
 1.244      100×

Protein purified on 10/27/2023 and assayed on 11/3/2023.
Data uploaded by Efraim and last updated on 2023-11-22 15:40:40.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.03195 0.05025
B 25.00 0.03330 0.04321
C 8.33 0.02295 0.01890
D 2.78 0.01620 0.01740
E 0.93 0.006000 0.007350 0.00570
F 0.31 0.001800 0.002250
G 0.10 0.0007500 0.001650 0.001350
H 0.03 0 0 0
 1.512      100×

Protein purified on 10/27/23 and assayed on 11/3/23.
Data uploaded by ahern798 and last updated on 2024-05-09 14:21:31.


Thermostability Assay Data

slope of C198M A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.00E+00 0.0006002 0.001050
B 48.3 0.0006000 0.0007500
C 45.7 0.0006000 0.0006000 0.0007500
D 42.4 0.0006000 0.0006000
E 37.7 0.001500 0.001650 0.001500
F 33.6 0.004050 0.003750 0.004350
G 31.3 0.006000 0.004350 0.005100
H 30.0 0.004950 0.005100 0.005100

Protein purified on 10/27/2023 and assayed on 11/3/2023.
Data uploaded by Efraim and last updated on 2023-11-17 10:55:40.


Reference Data for WT

slope of C198M A420 nm (min−1)
T (°C) 1 2 3
A 50.0 7.50E-04 7.50E-04
B 48.3 0 0 0
C 45.7 1.05E-03 7.50E-04 0
D 42.4 4.05E-03 3.45E-03 3.30E-03
E 37.7 1.76E-02 1.46E-02 2.01E-02
F 33.6 2.49E-02 2.40E-02 2.00E-02
G 31.3 2.55E-02 2.57E-02
H 30.0 2.61E-02 2.78E-02

Protein purified on 10/27/23 and assayed on 11/3/23.
Data uploaded by ahern798 and last updated on 2023-11-17 10:49:46.


Design Data

42.876

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.