D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant C198G

Expression Data

Yield (mg/mL): 0.9275

Kinetic Assay Data

**slope of C198G A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	6.00E-03	5.25E-03	5.55E-03
B	25.00	4.05E-03	4.35E-03	4.20E-03
C	8.33	3.45E-03	3.90E-03	3.60E-03
D	2.78	3.30E-03	3.00E-03	3.45E-03
E	0.93	1.80E-03	1.80E-03	1.95E-03
F	0.31	9.00E-04	1.20E-03
G	0.10	7.50E-04	7.50E-04	9.00E-04
H	0.03	6.00E-04	4.50E-04	9.00E-04

Yield (mg/mL): 0.9275 Dilution Factor: 100×

Protein purified on 10/27/23 and assayed on 11/3/23.
Data uploaded by Amanda2523 and last updated on 2023-11-17 10:24:04.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	7.07E-02	7.20E-02	6.98E-02
B	25.00	5.54E-02	5.30E-02	5.42E-02
C	8.33	4.85E-02	4.97E-02	4.94E-02
D	2.78	3.42E-02	3.24E-02	2.94E-02
E	0.93	2.04E-02	2.09E-02	1.64E-02
F	0.31	8.85E-03	8.55E-03	7.20E-03
G	0.10	4.80E-03	4.20E-03	3.30E-03
H	0.03		9.00E-04	7.50E-04

Yield (mg/mL): 2.03 Dilution Factor: 100×

Protein purified on 10/27/23 and assayed on 11/3/23.
Data uploaded by CaitlynR and last updated on 2023-11-17 10:17:43.

Thermostability Assay Data

**slope of C198G A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	30.0	1.92E-02	2.01E-02	2.07E-02
B	31.3	1.52E-02	1.56E-02	1.73E-02
C	33.6	2.10E-02	1.83E-02	2.04E-02
D	37.7	1.01E-02	9.15E-03	6.90E-03
E	42.4	1.50E-03	1.35E-03
F	45.7	6.00E-04		6.00E-04
G	48.3	1.65E-03	9.00E-04
H	50.0	6.00E-04		7.50E-04

Protein purified on 10/27/23 and assayed on 11/3/23.
Data uploaded by Amanda2523 and last updated on 2023-11-17 10:52:51.

Reference Data for WT

**slope of C198G A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	7.50E-04	6.00E-04	6.00E-04
B	48.3	7.50E-04		7.50E-04
C	45.7	1.05E-03	7.50E-04	6.00E-04
D	42.4	6.45E-03	6.15E-03	6.30E-03
E	37.7	3.36E-02	3.24E-02	2.55E-02
F	33.6	3.36E-02	3.03E-02	3.00E-02
G	31.3	5.16E-02	4.04E-02	4.07E-02
H	30.0	3.77E-02	4.31E-02	3.75E-02

Protein purified on 10/27/23 and assayed on 11/3/23.
Data uploaded by CaitlynR and last updated on 2023-11-17 10:48:03.

Design Data

Rosetta Score for C198G: 4.083

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.