Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 1.512

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00		0.03195	0.05025
	B	25.00		0.03330	0.04321
	C	8.33	0.02295	0.01890
	D	2.78	0.01620		0.01740
	E	0.93	0.006000	0.007350	0.00570
	F	0.31		0.001800	0.002250
	G	0.10	0.0007500	0.001650	0.001350
	H	0.03	0	0	0

Yield (mg/mL): 1.512     Dilution Factor:  100×

Download these data

Protein purified on 10/27/23 and assayed on 11/3/23.
Data uploaded by ahern798 and last updated on 2024-05-09 14:21:31.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	7.50E-04	7.50E-04
	B	48.3	0	0	0
	C	45.7	1.05E-03	7.50E-04	0
	D	42.4	4.05E-03	3.45E-03	3.30E-03
	E	37.7	1.76E-02	1.46E-02	2.01E-02
	F	33.6	2.49E-02	2.40E-02	2.00E-02
	G	31.3		2.55E-02	2.57E-02
	H	30.0		2.61E-02	2.78E-02

Download these data

Protein purified on 10/27/23 and assayed on 11/3/23.
Data uploaded by ahern798 and last updated on 2023-11-17 10:49:46.

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.