Raw Data for BglB Variant E129Q

Expression Data

Yield (mg/mL): 0.592

Kinetic Assay Data

slope of E129Q A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00
	B	25.00	7.20E-01	8.10E-01	8.70E-01
	C	8.33	7.20E-01	8.10E-01	8.30E-01
	D	2.78	6.70E-01	6.80E-01	7.00E-01
	E	0.93	3.50E-01	3.60E-01	4.40E-01
	F	0.31	9.00E-02	1.00E-01	1.00E-01
	G	0.10	3.00E-02	3.00E-02	4.00E-02
	H	0.03	2.69E-03	2.77E-03	2.03E-03

Yield (mg/mL): 0.592     Dilution Factor:  10×

Download these data

Protein purified on 9/29/2023 and assayed on 10/13/2023.
Data uploaded by pbuffington and last updated on 2023-11-17 11:22:21.

Reference Data for WT

Wild-type data not found. It may have been rejected in the curation process and deleted from the database.

Thermostability Assay Data

slope of E129Q A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	30.0	0.004809	0.004311
	B	31.3	0.002854	0.002889	0.002631
	C	33.6	0.002966	0.002871
	D	37.7	0.002331	0.001311	0.001783
	E	42.4	0.002203	0.001997	0.001551
	F	45.7	0.001594	0.003069	0.003703
	G	48.3		0.002477	0.002537
	H	50.0	0.00186	0.003111	0.004354

Download these data

Protein purified on 9/29/2023 and assayed on 10/20/2023.
Data uploaded by pbuffington and last updated on 2023-11-17 11:50:16.

Reference Data for WT

Wild-type data not found. It may have been rejected in the curation process and deleted from the database.

Design Data

Citations

The unpublished data above were collected by students from Loyola University Chicago. For inquiries, please contact Emma Feeney: <enielse@luc.edu>.