Raw Data for BglB Variant A88E
Expression Data
2.47
Kinetic Assay Data
| [S] (mm) | 1 | 2 | 3 | |||
|---|---|---|---|---|---|---|
| A | 75.00 | 4.30E-01 | 4.10E-01 | 4.80E-01 | ||
| B | 25.00 | 3.70E-01 | 3.60E-01 | 3.70E-01 | ||
| C | 8.33 | 1.90E-01 | 2.00E-01 | |||
| D | 2.78 | 9.00E-02 | 9.00E-02 | 8.00E-02 | ||
| E | 0.93 | 3.00E-02 | 3.00E-02 | 4.00E-02 | ||
| F | 0.31 | 1.00E-02 | 1.00E-02 | |||
| G | 0.10 | 7.05E-03 | 8.26E-03 | 6.44E-03 | ||
| H | 0.03 | 3.40E-03 | 2.49E-03 | 3.16E-03 |
Protein purified on 9/29/23 and assayed on 10/13/23.
Data uploaded by sharaddholakia and last updated on 2023-11-17 11:10:42.
Reference Data for WT
Wild-type data not found. It may have been rejected in the curation process and deleted from the database.
Thermostability Assay Data
| T (°C) | 1 | 2 | 3 | |||
|---|---|---|---|---|---|---|
| A | 50.0 | 1.12E-03 | 1.35E-03 | |||
| B | 48.3 | 1.65E-03 | 1.27E-03 | 1.54E-03 | ||
| C | 45.7 | 1.46E-03 | 3.43E-03 | 4.11E-04 | ||
| D | 42.4 | 2.25E-03 | 2.03E-03 | |||
| E | 37.7 | 3.81E-03 | 1.53E-03 | 2.38E-03 | ||
| F | 33.6 | 1.51E-03 | 1.78E-03 | 2.43E-03 | ||
| G | 31.3 | 2.13E-03 | 1.80E-03 | 1.16E-03 | ||
| H | 30.0 | 1.77E-03 | 1.39E-03 |
Reference Data for WT
Wild-type data not found. It may have been rejected in the curation process and deleted from the database.
Design Data
-1.442
Citations
The unpublished data above were collected by students from Loyola University Chicago. For inquiries, please contact Emma Feeney: <enielse@luc.edu>.