Raw Data for BglB Variant R76T

Expression Data

No expression

Kinetic Assay Data

slope of R76T A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	1.00E-02	3.50E-03
	B	25.00			4.88E-03
	C	8.33
	D	2.78	3.97E-03
	E	0.93		3.23E-03	2.43E-03
	F	0.31	3.63E-03	2.89E-03	2.54E-03
	G	0.10
	H	0.03	2.90E-03	3.16E-03	3.94E-03

Yield (mg/mL): 0.038     Dilution Factor:  10×

Download these data

Protein purified on 9/29/23 and assayed on 10/13/23.
Data uploaded by jstatho and last updated on 2023-12-01 12:55:44.

Reference Data for WT

Wild-type data not found. It may have been rejected in the curation process and deleted from the database.

Thermostability Assay Data

slope of R76T A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	1.62E-03	1.79E-03	1.95E-03
	B	48.3	1.62E-03		1.38E-03
	C	45.7	2.62E-03	2.49E-03
	D	42.4	1.65E-03	9.34E-04	2.00E-03
	E	37.7	2.08E-03		2.29E-03
	F	33.6		1.53E-03	1.44E-03
	G	31.3	7.54E-04	1.76E-03	1.30E-03
	H	30.0	8.83E-04	1.41E-03	2.69E-03

Download these data

Protein purified on 9/29/23 and assayed on 10/20/23.
Data uploaded by jstatho and last updated on 2023-11-17 12:20:27.

Reference Data for WT

Wild-type data not found. It may have been rejected in the curation process and deleted from the database.

Design Data

Citations

The unpublished data above were collected by students from Loyola University Chicago. For inquiries, please contact Emma Feeney: <enielse@luc.edu>.