Raw Data for BglB Variant M280I

Expression Data

Kinetic Assay Data

Plate 20201217-5

slope of M280I A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	0.0314206	0.0286715	0.0332982
	B	18.75	0.0252394	0.0228012	0.0243976
	C	4.69	0.0147636	0.0152485	0.0146036
	D	1.17	0.00677333	0.00619333	0.00612364
	E	0.29	0.00187818	0.00177394	0.00180182
	F	0.07	0.000528485	0.000497576	0.000466061
	G	0.02		0.000135152	0.000140606
	H	0.00	4.97E-05	5.76E-05	5.45E-05

Yield / A_{280 nm}: 0.54     Dilution Factor:  100×

Download these data

Protein purified on 11/17/19 and assayed on 11/17/19.
Data uploaded by peishanh and last updated on 2022-06-27 07:04:03.

Reference Data for WT

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	7.42E-02	6.73E-02	6.85E-02
	B	18.75	5.73E-02	4.89E-02	5.15E-02
	C	4.69	3.26E-02	3.20E-02	3.33E-02
	D	1.17	1.31E-02	1.36E-02	1.28E-02
	E	0.29	3.92E-03	4.05E-03	3.94E-03
	F	0.07	1.06E-03	9.73E-04	8.26E-04
	G	0.02	2.87E-04	2.56E-04	3.15E-04
	H	0.00	7.88E-05	8.12E-05	6.85E-05

Yield / A_{280 nm}: 0.879     Dilution Factor:  100×

Download these data

Protein purified on 11/17/19 and assayed on 11/17/19.
Data uploaded by peishanh and last updated on 2022-06-27 07:04:03.

Citations

The above data have been published in the following journal article(s):

• • Huang, P.; Chu, S.K.; Frizzo, H.N.; Connolly, M.P.; Caster, R.W.; & Siegel, J. B. (2020) “Evaluating Protein Engineering Thermostability Prediction Tools Using an Independently Generated Dataset..” ACS Omega 5(12), 6487–6493.