D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant W409N

Expression Data

Yield (mg/mL): 8.273

Kinetic Assay Data

**slope of W409N A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.89E-01	1.77E-01	1.71E-01
B	25.00	1.41E-01	1.34E-01	1.34E-01
C	8.33	1.22E-01	1.15E-01	9.57E-02
D	2.78	7.41E-02	9.57E-02	5.96E-02
E	0.93	5.04E-02	4.85E-02	4.83E-02
F	0.31	2.19E-02	1.85E-02	2.75E-02
G	0.10	7.95E-03	1.04E-02	8.10E-03
H	0.03	0	0

Yield (mg/mL): 8.273 Dilution Factor: 100×

Protein purified on 10/27/23 and assayed on 11/3/23.
Data uploaded by arusa001 and last updated on 2023-11-30 12:10:05.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	3.30E-01	3.29E-01	3.23E-01
B	25.00	2.88E-01	2.74E-01	2.75E-01
C	8.33	2.24E-01	2.14E-01	2.14E-01
D	2.78	1.28E-01	1.24E-01	1.27E-01
E	0.93	6.06E-02	5.64E-02	5.88E-02
F	0.31	2.19E-02	2.19E-02	2.24E-02
G	0.10	8.10E-03	8.10E-03	8.10E-03
H	0.03	0	0	0

Yield (mg/mL): 6.244 Dilution Factor: 100×

Protein purified on 10/27/2023 and assayed on 11/3/2023.
Data uploaded by jrodr1379 and last updated on 2023-11-17 14:21:54.

Thermostability Assay Data

**slope of W409N A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	6.00E-04	7.50E-04	7.50E-04
B	48.3	1.20E-03	0	7.50E-04
C	45.7	7.50E-04	9.00E-04	9.00E-04
D	42.4	3.15E-03	3.15E-03
E	37.7	9.84E-02	7.82E-02	8.19E-02
F	33.6	1.09E-01	1.03E-01	1.05E-01
G	31.3	9.24E-02	9.17E-02	8.88E-02
H	30.0	9.47E-02	9.71E-02	1.04E-01

Protein purified on 10/27/23 and assayed on 11/3/23.
Data uploaded by arusa001 and last updated on 2023-11-17 15:06:26.

Reference Data for WT

**slope of W409N A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0		0	0
B	48.3	7.50E-04		6.00E-04
C	45.7	6.00E-04	6.00E-04	7.50E-04
D	42.4	1.83E-02	1.62E-02	1.62E-02
E	37.7	1.59E-01	1.54E-01	1.51E-01
F	33.6	1.88E-01	1.66E-01	1.72E-01
G	31.3	1.89E-01	1.61E-01	1.37E-01
H	30.0		1.42E-01	1.32E-01

Protein purified on 10/27/2023 and assayed on 11/3/2023.
Data uploaded by jrodr1379 and last updated on 2023-11-17 14:27:37.

Design Data

Rosetta Score for W409N: 1.97

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.