D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant W409A

Expression Data

Yield (mg/mL): 4.657

Kinetic Assay Data

**slope of W409A A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.72E-01	1.61E-01	1.72E-01
B	25.00	1.16E-01	1.17E-01	1.21E-01
C	8.33	9.48E-02	9.77E-02
D	2.78	6.29E-02	7.19E-02	6.60E-02
E	0.93	3.18E-02	3.42E-02	3.62E-02
F	0.31		1.34E-02	1.35E-02
G	0.10	4.80E-03	4.80E-03	4.95E-03
H	0.03	7.50E-04	4.50E-04	6.00E-04

Yield (mg/mL): 4.657 Dilution Factor: 100×

Protein purified on 10/27/23 and assayed on 11/3/23.
Data uploaded by JG2002 and last updated on 2023-11-17 14:51:18.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	9.02E-02	8.79E-02	9.23E-02
B	25.00	6.96E-02	7.31E-02	7.19E-02
C	8.33	4.73E-02	5.81E-02	6.23E-02
D	2.78		3.29E-02	3.57E-02
E	0.93	1.29E-02	1.67E-02	1.82E-02
F	0.31	6.00E-03	5.25E-03	5.55E-03
G	0.10	2.25E-03	2.25E-03	2.40E-03
H	0.03	6.00E-04	7.50E-04	6.00E-04

Yield (mg/mL): 2.821 Dilution Factor: 100×

Protein purified on 10/27/2023 and assayed on 11/3/2023.
Data uploaded by jordangarcia0221 and last updated on 2023-11-17 14:13:26.

Thermostability Assay Data

**slope of W409A A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0		6.00E-04	6.00E-04
B	48.3	0		0
C	45.7	7.50E-04	7.50E-04
D	42.4	6.15E-03	5.25E-03	5.25E-03
E	37.7	3.45E-02	3.87E-02	3.95E-02
F	33.6	3.87E-02	3.95E-02	3.44E-02
G	31.3	4.38E-02	4.56E-02
H	30.0	4.44E-02	4.44E-02	4.17E-02

Protein purified on 10/27/23 and assayed on 11/3/23.
Data uploaded by JG2002 and last updated on 2023-11-17 14:59:57.

Reference Data for WT

**slope of W409A A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	7.50E-04		7.50E-04
B	48.3	6.00E-04	4.50E-04
C	45.7	6.00E-04	6.00E-04	7.50E-04
D	42.4	4.95E-03	5.70E-03	4.95E-03
E	37.7	3.57E-02	3.35E-02	3.24E-02
F	33.6	3.86E-02	3.71E-02	3.45E-02
G	31.3	3.90E-02	3.45E-02	3.53E-02
H	30.0	3.06E-02	3.06E-02	2.78E-02

Protein purified on 10/27/2023 and assayed on 11/3/2023.
Data uploaded by jordangarcia0221 and last updated on 2023-11-17 14:16:51.

Design Data

Rosetta Score for W409A: 3.623

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.