D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant W409I

Expression Data

Yield (mg/mL): 1.127

Kinetic Assay Data

**slope of W409I A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	2.12E-02	1.97E-02	1.97E-02
B	25.00	1.62E-02	1.67E-02	1.71E-02
C	8.33		1.07E-02	1.22E-02
D	2.78	8.25E-03	8.40E-03	8.85E-03
E	0.93	4.20E-03	3.45E-03	3.90E-03
F	0.31	1.65E-03	2.10E-03	1.65E-03
G	0.10	1.20E-03	9.00E-04	1.05E-03
H	0.03	7.50E-04		7.50E-04

Yield (mg/mL): 1.127 Dilution Factor: 10×

Protein purified on 10/27/2023 and assayed on 11/3/2023.
Data uploaded by Kevyu0210 and last updated on 2023-11-17 14:29:45.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	9.02E-02	8.79E-02	9.23E-02
B	25.00	6.96E-02	7.31E-02	7.19E-02
C	8.33	4.73E-02	5.81E-02	6.23E-02
D	2.78		3.29E-02	3.57E-02
E	0.93	1.29E-02	1.67E-02	1.82E-02
F	0.31	6.00E-03	5.25E-03	5.55E-03
G	0.10	2.25E-03	2.25E-03	2.40E-03
H	0.03	6.00E-04	7.50E-04	6.00E-04

Yield (mg/mL): 2.821 Dilution Factor: 100×

Protein purified on 10/27/2023 and assayed on 11/3/2023.
Data uploaded by jordangarcia0221 and last updated on 2023-11-17 14:13:26.

Thermostability Assay Data

**slope of W409I A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	1.05E-03	4.50E-04	0
B	48.3	1.05E-03	6.00E-04	0
C	45.7		4.50E-04	4.50E-04
D	42.4	1.50E-03	1.80E-03	1.65E-03
E	37.7	6.90E-03	6.90E-03	7.35E-03
F	33.6	8.10E-03	8.10E-03	7.35E-03
G	31.3	7.80E-03	8.70E-03	7.05E-03
H	30.0		8.25E-03	7.50E-03

Protein purified on 10/27/2023 and assayed on 11/3/2023.
Data uploaded by Kevyu0210 and last updated on 2024-05-09 12:21:18.

Reference Data for WT

**slope of W409I A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	7.50E-04		7.50E-04
B	48.3	6.00E-04	4.50E-04
C	45.7	6.00E-04	6.00E-04	7.50E-04
D	42.4	4.95E-03	5.70E-03	4.95E-03
E	37.7	3.57E-02	3.35E-02	3.24E-02
F	33.6	3.86E-02	3.71E-02	3.45E-02
G	31.3	3.90E-02	3.45E-02	3.53E-02
H	30.0	3.06E-02	3.06E-02	2.78E-02

Protein purified on 10/27/2023 and assayed on 11/3/2023.
Data uploaded by jordangarcia0221 and last updated on 2023-11-17 14:16:51.

Design Data

Rosetta Score for W409I: 10.658

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.