D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant W409V

Expression Data

Yield (mg/mL): 4.173

Kinetic Assay Data

**slope of W409V A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.60E-01	1.60E-01	1.62E-01
B	25.00	1.03E-01	1.18E-01	1.12E-01
C	8.33	7.49E-02	8.36E-02	9.47E-02
D	2.78	4.37E-02	4.29E-02	3.89E-02
E	0.93	1.88E-02	1.79E-02	1.94E-02
F	0.31	7.80E-03	7.50E-03	7.65E-03
G	0.10	2.85E-03	2.70E-03	2.85E-03
H	0.03	0		0

Yield (mg/mL): 4.173 Dilution Factor: 100×

Protein purified on 10/27/23 and assayed on 11/3/23.
Data uploaded by Mlitk001 and last updated on 2023-11-17 14:41:35.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	9.02E-02	8.79E-02	9.23E-02
B	25.00	6.96E-02	7.31E-02	7.19E-02
C	8.33	4.73E-02	5.81E-02	6.23E-02
D	2.78		3.29E-02	3.57E-02
E	0.93	1.29E-02	1.67E-02	1.82E-02
F	0.31	6.00E-03	5.25E-03	5.55E-03
G	0.10	2.25E-03	2.25E-03	2.40E-03
H	0.03	6.00E-04	7.50E-04	6.00E-04

Yield (mg/mL): 2.821 Dilution Factor: 100×

Protein purified on 10/27/2023 and assayed on 11/3/2023.
Data uploaded by jordangarcia0221 and last updated on 2023-11-17 14:13:26.

Thermostability Assay Data

**slope of W409V A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	9.00E-04	9.00E-04
B	48.3		1.20E-03	1.05E-03
C	45.7	7.50E-04	9.00E-04	7.50E-04
D	42.4	3.60E-03	3.75E-03	4.20E-03
E	37.7	2.24E-02	2.25E-02	2.22E-02
F	33.6	3.26E-02	2.94E-02	2.82E-02
G	31.3	2.60E-02	2.79E-02	2.09E-02
H	30.0	1.38E-02	1.23E-02

Protein purified on 10/27/23 and assayed on 11/3/23.
Data uploaded by Mlitk001 and last updated on 2023-11-17 14:59:17.

Reference Data for WT

**slope of W409V A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	7.50E-04		7.50E-04
B	48.3	6.00E-04	4.50E-04
C	45.7	6.00E-04	6.00E-04	7.50E-04
D	42.4	4.95E-03	5.70E-03	4.95E-03
E	37.7	3.57E-02	3.35E-02	3.24E-02
F	33.6	3.86E-02	3.71E-02	3.45E-02
G	31.3	3.90E-02	3.45E-02	3.53E-02
H	30.0	3.06E-02	3.06E-02	2.78E-02

Protein purified on 10/27/2023 and assayed on 11/3/2023.
Data uploaded by jordangarcia0221 and last updated on 2023-11-17 14:16:51.

Design Data

Rosetta Score for W409V: 4.97

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.