Raw Data for BglB Variant E340A

Expression Data

SDS-PAGE
 1

Kinetic Assay Data

slope of E340A A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.1736 0.1857 0.1939
B 25.00 0.1480 0.1598 0.1644
C 8.33 0.1293 0.1266 0.1241
D 2.78 0.1008 0.09985 0.09115
E 0.93 0.05550 0.05205 0.04415
F 0.31 0.02275 0.02050 0.01790
G 0.10 0.009700 0.008450 0.006850
H 0.03 0.003700 0.004300 0.002550
 1      100×

Protein purified on 11/8/2023 and assayed on 11/15/2023.
Data uploaded by kyleh and last updated on 2023-11-29 11:53:43.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.1618 0.1632 0.1467
B 25.00 0.1251 0.1295 0.1422
C 8.33 0.09470 0.08805 0.09315
D 2.78 0.07140 0.06960 0.07660
E 0.93 0.03465 0.03455 0.04030
F 0.31 0.01480 0.01450 0.01650
G 0.10 0.005650 0.005600 0.006700
H 0.03 0.002450 0.002000 0.002450
 1.4      100×

Protein purified on 11/8/2023 and assayed on 11/15/2023.
Data uploaded by joshhyun and last updated on 2023-11-29 11:59:56.


Thermostability Assay Data

slope of E340A A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.001100 0.0006000
B 48.3 0.0009000 0.002800 0.001400
C 45.7 0.001000 0.0008000 0.0009000
D 42.4 0.008200 0.008100 0.008100
E 37.7 0.1195 0.1177 0.1092
F 33.6 0.1771 0.1784 0.1714
G 31.3 0.1570 0.1765 0.1771
H 30.0 0.2029 0.1684 0.1931

Protein purified on 11/8/2023 and assayed on 11/15/2023.
Data uploaded by kyleh and last updated on 2023-11-29 12:07:12.


Reference Data for WT

slope of E340A A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.0006000 0.0005000
B 48.3 0.001000 0.0007001 0.0006000
C 45.7 0.001900 0.001800
D 42.4 0.06880 0.06880 0.06040
E 37.7 0.1368 0.1501 0.1249
F 33.6 0.1500 0.1335 0.1107
G 31.3 0.1380 0.1077 0.1246
H 30.0 0.1141 0.1182 0.1101

Protein purified on 11/8/2023 and assayed on 11/15/2023.
Data uploaded by joshhyun and last updated on 2023-11-29 12:05:16.


Design Data

3.972

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Justin B. Siegel: <jbsiegel@ucdavis.edu>.