Raw Data for BglB Variant W11F

Expression Data

Kinetic Assay Data

Plate 20201217-5

slope of W11F A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	0.0757527	0.0776303	0.0735345
	B	18.75	0.0625921	0.0667697	0.0538424
	C	4.69	0.0338958	0.038957	0.0382158
	D	1.17	0.0163418	0.0160473	0.0155558
	E	0.29	0.0051497	0.0048497	0.00438424
	F	0.07	0.00138485	0.00129091	0.0012503
	G	0.02	0.000355758	0.000351515	0.000387273
	H	0.00	0.000108485	0.000110303

Yield / A_{280 nm}: 0.711     Dilution Factor:  100×

Download these data

Protein purified on 11/17/19 and assayed on 11/17/19.
Data uploaded by peishanh and last updated on 2022-06-27 07:04:03.

Reference Data for WT

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	7.42E-02	6.73E-02	6.85E-02
	B	18.75	5.73E-02	4.89E-02	5.15E-02
	C	4.69	3.26E-02	3.20E-02	3.33E-02
	D	1.17	1.31E-02	1.36E-02	1.28E-02
	E	0.29	3.92E-03	4.05E-03	3.94E-03
	F	0.07	1.06E-03	9.73E-04	8.26E-04
	G	0.02	2.87E-04	2.56E-04	3.15E-04
	H	0.00	7.88E-05	8.12E-05	6.85E-05

Yield / A_{280 nm}: 0.879     Dilution Factor:  100×

Download these data

Protein purified on 11/17/19 and assayed on 11/17/19.
Data uploaded by peishanh and last updated on 2022-06-27 07:04:03.

Citations

The above data have been published in the following journal article(s):

• • Huang, P.; Chu, S.K.; Frizzo, H.N.; Connolly, M.P.; Caster, R.W.; & Siegel, J. B. (2020) “Evaluating Protein Engineering Thermostability Prediction Tools Using an Independently Generated Dataset..” ACS Omega 5(12), 6487–6493.