Raw Data for BglB Variant N220F

Expression Data

SDS-PAGE
 1.8

Kinetic Assay Data

slope of N220F A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.3676 0.3703
B 25.00 0.3388 0.3430 0.3434
C 8.33 0.3158 0.3233 0.2992
D 2.78 0.2755 0.2174 0.2303
E 0.93 0.1796 0.1861
F 0.31 0.07535 0.06475
G 0.10 0.0006000 0.0009500
H 0.03 0 0
 1.8      100×

Protein purified on 11/8/2023 and assayed on 11/15/2023.
Data uploaded by eovando and last updated on 2023-11-29 11:59:21.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.2026 0.2552 0.3866
B 25.00 0.2959 0.3224 0.2882
C 8.33 0.2211 0.2181 0.2233
D 2.78 0.1429 0.1312 0.1342
E 0.93 0.06365 0.06025 0.0582
F 0.31 0.01305 0.0193 0.01585
G 0.10 0.0116 0.0089 0.00905
H 0.03 0.00855 0.0106 0.0077
 1.4      100×

Protein purified on 11/8/2023 and assayed on 11/15/2023.
Data uploaded by rrachel11 and last updated on 2023-11-29 11:59:31.


Thermostability Assay Data

slope of N220F A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.0008000 0.0006001
B 48.3 0.0008000 0.0006000 0
C 45.7 0.001100 0.001500 0.001300
D 42.4 0.01280 0.01200 0.01160
E 37.7 0.1141 0.1016 0.1461
F 33.6 0.1539 0.1515 0.1417
G 31.3 0.1615 0.1469 0.1479
H 30.0 0.1794 0.1705 0.1786

Protein purified on 11/8/2023 and assayed on 11/15/2023.
Data uploaded by eovando and last updated on 2023-11-29 12:08:44.


Reference Data for WT

slope of N220F A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0 0
B 48.3 0.0007 0.0005
C 45.7 0.0008 0.0011 0.001
D 42.4 0.0217 0.0212 0.0252
E 37.7 0.1001 0.0943
F 33.6 0.0961 0.0947
G 31.3 0.1006 0.0981 0.0941
H 30.0 0.0902 0.0968 0.0784

Protein purified on 11/8/2023 and assayed on 11/15/2023.
Data uploaded by rrachel11 and last updated on 2023-11-29 12:02:38.


Design Data

-1.979

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.