D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant N404M

Expression Data

Yield (mg/mL): 4

Kinetic Assay Data

**slope of N404M A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.01640	0.01650	0.01670
B	25.00	0.01345	0.01185	0.01095
C	8.33	0.007750	0.008450	0.008450
D	2.78	0.004050	0.004000	0.004250
E	0.93	0.001550	0.001700	0.001850
F	0.31		0.0009000	0.001000
G	0.10	0.0006500	0.0008000	0.0004500
H	0.03	0.0005500	0.0005500

Yield (mg/mL): 4 Dilution Factor: 100×

Protein purified on 11/8/2023 and assayed on 11/15/2023.
Data uploaded by bhrala and last updated on 2023-11-29 11:59:50.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	3.14E-01	3.22E-01	3.12E-01
B	25.00	2.81E-01	2.81E-01	2.80E-01
C	8.33	2.43E-01	2.13E-01	2.25E-01
D	2.78	1.50E-01	1.40E-01	1.46E-01
E	0.93	6.52E-02	6.18E-02	6.41E-02
F	0.31	2.95E-02	2.73E-02	3.32E-02
G	0.10	1.35E-02	1.24E-02	1.29E-02
H	0.03	4.65E-03	4.50E-03	5.00E-03

Yield (mg/mL): 2.5 Dilution Factor: 100×

Protein purified on 11/8/2023 and assayed on 11/15/2023.
Data uploaded by TaylonB and last updated on 2023-11-29 11:59:50.

Thermostability Assay Data

**slope of N404M A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	0.001700	0	0.0006000
B	48.3	0		0
C	45.7	0.0007000	0	0.0005000
D	42.4	0.001500	0.001700	0.001700
E	37.7	0.005700	0.005700	0.005500
F	33.6	0.006000	0.006000	0.006600
G	31.3	0.006400	0.006700	0.006200
H	30.0	0.006100	0.006100	0.006500

Protein purified on 11/8/2023 and assayed on 11/15/2023.
Data uploaded by bhrala and last updated on 2023-11-29 12:04:28.

Reference Data for WT

**slope of N404M A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	1.44E-02	6.00E-04	5.60E-03
B	48.3	6.00E-04		5.00E-04
C	45.7	1.30E-03	1.00E-03	1.50E-03
D	42.4		4.78E-02	4.94E-02
E	37.7	1.80E-01	1.82E-01	1.69E-01
F	33.6	2.11E-01	2.00E-01	2.06E-01
G	31.3	2.13E-01	2.18E-01	2.15E-01
H	30.0	1.56E-01	1.67E-01	1.65E-01

Protein purified on 11/8/2023 and assayed on 11/15/2023.
Data uploaded by TaylonB and last updated on 2023-11-29 12:04:53.

Design Data

Rosetta Score for N404M: -3.373

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.