Raw Data for BglB Variant R240A

Expression Data

SDS-PAGE
 1.1

Kinetic Assay Data

slope of R240A A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.1870 0.1968 0.2005
B 25.00 0.1245 0.1269 0.1337
C 8.33 0.08204 0.07705 0.08150
D 2.78 0.05055 0.05185 0.04730
E 0.93 0.02230 0.02360 0.01990
F 0.31 0.007750 0.008650 0.006750
G 0.10 0.002650 0.003050 0.002650
H 0.03 0.001250 0.001500 0.001350
 1.1      100×

Protein purified on 11/8/2023 and assayed on 11/15/2023.
Data uploaded by Dchander and last updated on 2023-11-29 12:15:57.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.2098 0.1786 0.1961
B 25.00 0.1684 0.1633 0.1804
C 8.33 0.1296 0.1325 0.1273
D 2.78 0.08390 0.07235 0.07640
E 0.93 0.04800 0.03750 0.03875
F 0.31 0.01840 0.01460 0.01415
G 0.10 0.006900 0.005250 0.005100
H 0.03 0.003050 0.002250 0.002150
 1.7      100×

Protein purified on 11/8/2023 and assayed on 11/15/2023.
Data uploaded by isaacmathews and last updated on 2023-11-29 12:00:34.


Thermostability Assay Data

slope of R240A A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.0007000 0.0007000
B 48.3 0.0006000 0.0008000
C 45.7 0.0007000 0.001000 0.0009000
D 42.4 0.01060 0.01100 0.01050
E 37.7 0.09140 0.08580 0.1006
F 33.6 0.1235 0.1267 0.1291
G 31.3 0.1375 0.1366 0.1324
H 30.0 0.1555 0.1465 0.1521

Protein purified on 11/8/2023 and assayed on 11/15/2023.
Data uploaded by Dchander and last updated on 2023-11-29 12:20:22.


Reference Data for WT

slope of R240A A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.001100 0.0006000 0.001400
B 48.3 0.0008000 0.0007000
C 45.7 0.001300 0.001200
D 42.4 0.04820 0.04860 0.04990
E 37.7 0.1937 0.1847 0.1843
F 33.6 0.1940 0.1955 0.2040
G 31.3 0.2268 0.2221 0.2095
H 30.0 0.2272 0.2121 0.2062

Protein purified on 11/8/2023 and assayed on 11/15/2023.
Data uploaded by isaacmathews and last updated on 2023-11-29 12:06:49.


Design Data

2.307

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.