Raw Data for BglB Variant R240A

Expression Data

SDS-PAGE
 3.3

Kinetic Assay Data

slope of R240A A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 4.98E-01 5.15E-01 5.29E-01
B 25.00 3.18E-01 3.43E-01 3.72E-01
C 8.33 2.30E-01 2.52E-01 2.40E-01
D 2.78 1.49E-01 1.49E-01 1.46E-01
E 0.93 5.46E-02 5.69E-02 5.86E-02
F 0.31 1.85E-02 1.97E-02 2.15E-02
G 0.10 6.15E-03 6.90E-03 7.50E-03
H 0.03 3.00E-03 3.05E-03 3.20E-03
 3.3      100×

Protein purified on and assayed on .
Data uploaded by yraghupa and last updated on 2023-11-29 12:24:36.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.2098 0.1786 0.1961
B 25.00 0.1684 0.1633 0.1804
C 8.33 0.1296 0.1325 0.1273
D 2.78 0.08390 0.07235 0.07640
E 0.93 0.04800 0.03750 0.03875
F 0.31 0.01840 0.01460 0.01415
G 0.10 0.006900 0.005250 0.005100
H 0.03 0.003050 0.002250 0.002150
 1.7      100×

Protein purified on 11/8/2023 and assayed on 11/15/2023.
Data uploaded by isaacmathews and last updated on 2023-11-29 12:00:34.


Thermostability Assay Data

slope of R240A A420 nm (min−1)
T (°C) 1 2 3
A 50.0 8.00E-04 0 5.00E-04
B 48.3 8.00E-04 7.00E-04
C 45.7 8.00E-04 7.00E-04 1.10E-03
D 42.4 1.81E-02 2.16E-02 2.36E-02
E 37.7 2.74E-01 2.79E-01 2.65E-01
F 33.6 3.62E-01 3.40E-01 3.32E-01
G 31.3 3.58E-01 3.63E-01
H 30.0 3.72E-01 3.87E-01 3.82E-01

Protein purified on and assayed on .
Data uploaded by yraghupa and last updated on 2023-11-29 12:22:36.


Reference Data for WT

slope of R240A A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.001100 0.0006000 0.001400
B 48.3 0.0008000 0.0007000
C 45.7 0.001300 0.001200
D 42.4 0.04820 0.04860 0.04990
E 37.7 0.1937 0.1847 0.1843
F 33.6 0.1940 0.1955 0.2040
G 31.3 0.2268 0.2221 0.2095
H 30.0 0.2272 0.2121 0.2062

Protein purified on 11/8/2023 and assayed on 11/15/2023.
Data uploaded by isaacmathews and last updated on 2023-11-29 12:06:49.


Design Data

2.297

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.