D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant F77W

Expression Data

Yield (mg/mL): 0.384

Kinetic Assay Data

Plate 20201217-tali2

**slope of F77W A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00		0.000566667	0.000481212
B	18.75		0.000202424	0.000190303
C	4.69		8.30E-05	7.76E-05
D	1.17		4.61E-05	3.39E-05
E	0.29		0	3.21E-05
F	0.07	4.59E-04	2.91E-05	0.00013697
G	0.02
H	0.00

Yield / A_{280 nm}: 0.384 Dilution Factor: 100×

Protein purified on 2001.01.20 and assayed on 2001.01.20.
Data uploaded by vater and last updated on 2022-06-27 07:04:03.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.0102655	0.00766848
B	18.75	0.00849818	0.00768848
C	4.69	0.0049703	0.00447758
D	1.17	0.00229455	0.00184606
E	0.29	0.000653939	0.000593939
F	0.07	0.000184242	0.000178788
G	0.02	1.37E-04		0.000159394
H	0.00	1.70E-05		2.30E-05

Yield / A_{280 nm}: 0.102 Dilution Factor: 50×

Protein purified on 2001.01.20 and assayed on 2001.01.20.
Data uploaded by vater and last updated on 2022-06-27 07:04:03.

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.