Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 0.9

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	0.0696	0.0785	0.0693
	B	25.00	0.0523	0.0540	0.0563
	C	8.33	0.0550	0.0555	0.0512
	D	2.78	0.0389	0.0376	0.0368
	E	0.93	0.02680	0.02435	0.01735
	F	0.31	0.00890	0.01100	0.00830
	G	0.10	0.00385		0.00345
	H	0.03	0.000200	0	0.000300

Yield (mg/mL): 0.9     Dilution Factor:  100×

Download these data

Protein purified on 11/8/2023 and assayed on 11/15/2023.
Data uploaded by manyarohloff and last updated on 2023-11-29 15:11:33.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	0.00040	0.0004000
	B	48.3	0.0006000	0.000400	0.0005000
	C	45.7	0.001100	0.000900	0.001000
	D	42.4	0.02560	0.02820	0.03290
	E	37.7	0.0770	0.0776
	F	33.6	0.1330	0.1150	0.1180
	G	31.3
	H	30.0

Download these data

Protein purified on 11/8/2023 and assayed on 11/15/2023.
Data uploaded by manyarohloff and last updated on 2023-12-21 18:39:22.

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.