D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant W407H

Expression Data

Yield (mg/mL): 0.5

Kinetic Assay Data

**slope of W407H A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.15E-03	1.20E-03	1.15E-03
B	25.00	7.00E-04	8.50E-04	8.00E-04
C	8.33	6.00E-04	4.50E-04	4.50E-04
D	2.78	3.00E-04		2.50E-04
E	0.93	3.50E-04		3.00E-04
F	0.31	2.50E-04	3.00E-04	3.50E-04
G	0.10	2.50E-04	2.00E-04	3.00E-04
H	0.03		0	0

Yield (mg/mL): 0.5 Dilution Factor: 10×

Protein purified on 11/8/2023 and assayed on 11/15/2023.
Data uploaded by IzzyG and last updated on 2023-11-29 14:59:24.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.3034	0.3265	0.3365
B	25.00	0.2429	0.2700	0.2605
C	8.33	0.2028	0.2426	0.2283
D	2.78	0.1896	0.1578	0.1641
E	0.93	0.12050	0.07395	0.09180
F	0.31	0.04890	0.02885	0.04095
G	0.10	0.02400	0.01120	0.01795
H	0.03	0.000200	0.000150	0.000300

Yield (mg/mL): 1.5 Dilution Factor: 100×

Protein purified on 11/8/2023 and assayed on 11/15/2023.
Data uploaded by Beni and last updated on 2023-11-29 14:50:49.

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.